Platelet Adhesion and Aggregation under Dynamic Shear

Platelet Adhesion and Aggregation under Dynamic Shear 

Dissolve 500 mg collagen type I from bovine Achille s tendon into 200 ml of 0.5 mol/L acetic acid in water, pH 2.8 

Centrifuge the homogenate at 200 x g for 10 min, collect supernatant, and measure collagen concentration by a modified Lowry analysis. 

Coat glass coverslips with 200 xl of fibrillar collagen I suspension on all but first 10 mm of the slide length (coated area = 12.7 x 23), and place in a humid environment at 37 °C for 45 min. 

Rinse excess collagen with 10 ml of D-PBS maintained at 37 °C before assembly into the flow chamber. 

---