Platelet-activating factor , binding sites

Marcheselli, V. L. Rossowska, M. J. Domingo, M. T. Braquet, P Bazan, N. G. Distinct platelet-activating factor binding sites in synaptic endings and in intracellular membranes of rat cerebral cortex. J. Biol. Chem., 1990, 265 9140-9145. 

ChsuLY, Tsai YM, Cheng JR. Photoaflinity Idling of platelet activating factor binding sites in rabbit platelet membranes. Biocfaem BiophysResCommun 1989 161 1070-1076... 

Sandwich-type sensing platforms are also nsed widely in electrochemical aptasensors (Willner and Zayats, 2007), especially for common model molecnles such as a-thrombin (Mir et al., 2006 Polsky et al., 2006 Centi et al., 2007) and platelet-derived growth factor (PDGF) (Zhou et al., 2007), which possess two active aptamer-binding sites. This type of sensor usually... 

Platelet membrane phosphatidylserine is critical to the formation of the tenase complex since on its surface activated factor VIII (Villa) generates a high-afflnity binding site for activated factor IX (IXa) in the presence of calcium. Subsequently, this complex activates factor X (2, 13). Platelet membrane phosphatidylserine similarly anchors activated factor V (Va), favoring the calcium-dependent binding of activated factor X (Xa). The prothrombinase complex is generated on the surface of the anionic platelet membrane phosphatidylserine when factor Va binds prothrombin. The prothrombinase complex cleaves prothrombin to produce thrombin, which has a multifunctional role (14). 

Platelet activation leads to the formation of platelet-derived microparticles derived from the platelet surface. These microvesicles typically account for 25% to 30% of platelet procoagulant activity and factor V binding sites (34,39),... 

Factor Xa binds to the phospholipid membranes released by platelet aggregation. Factor Xa cleaves the zymogen, prothrombin, at two specific sites to produce the active enzyme, thrombin. 

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