Plasmids mapping

Fig. 1. Yeast surface display elements (A) Plasmid map of the yeast surface display vector pCT302 with a single-chain T cell receptor (scTCR) cloned in as a fusion with the yeast mating agglutinin protein Aga-2. Map was prepared with the help of PlasMapper (33). The open reading frame containing Aga-2 and the scTCR is expanded to show the important elements and restriction sites. (B) Diagram of a bioreceptor displayed on the surface of yeast fused to Aga-2. A detection scheme involving biotinylated ligand bound to the bioreceptor and fluorescent streptavidin may be used to analyze bioreceptor libraries on the surface of yeast.

Dong, X., Stothard, P., Forsythe, I. J. and Wishart, D. S. (2004) PlasMapper a web server for drawing and auto-annotat-ing plasmid maps. Nucleic Acids Res 32, W660-W664... 

Fig 1 Plasmid maps of the three expression plasmids used to express Biotin Carboxyl Carrier Protein without transit peptide, BCCP with transit peptide and Biotin Carboxylase without transit peptide. 

Plasmid Vectors for Facile Introduction of Passenger DNA and Selection of Recombinants. The map of a commonly used plasmid vector, pUC19 (7), is shown in Figure 2. Three parts of the vector are key to its utility. The origin sequence, oh, allows the repHcation of plasmid DNA in high copy number relative to the chromosome. A gene, amp, encoding the enzyme beta-lactamase, which hydrolyzes penicillin compounds, allows... 

Fig. 2. (a) Map of pUC19, a commonly used plasmid vector where the numbers correspond to the positions of the various restriction enzyme cuts and (b) nucleic acid composition of pUC19 from position 393 (5 -end) through position 469 (3 -end) (5,7). 

Fig. 2. Schematic representation of the Ti-plasmid of Agrobacterium, showing the genes involved in transformation of host plants. Note that the genetic map is not to scale in reality, the T-region makes up about 10% of the total plasmid genome (from Bryant, 1988).

Fig.l. Gene maps of recombinant plasmids pTOH, pTTOH, pEOH, and pETOH. Abbreviations Pnc, trc promoter Px7, T7 and lac hybrid promoter Tat, twin-arginme TorA signal sequence of TMAO reductase OPH, organophosphoms hydrolase gene Hisg, hexa-histidine affinity tag. 

Figure 1. Restriction nuclease maps of the pern carrying plasmids.

Don RH, JM Pemberton (1985) Genetic and physical map of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pJP4. J Bacteriol 161 466-468. 

Horinouchi, S. and Weisblum, B. (1982) Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies. Journal of... 

Fig. 2. Partial restriction enzyme map of plasmid pAMD 101. The blackened segment shows A. bronchisepticus DNA of 1.2 kb...

A restriction map of plasmid pBR322 indicating the positions of its antibiotic resistance genes and the sites of nucleotide sequences recognized by specific restriction endonucleases. 

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