Plant lipidomics development

As the development of lipidomics field, mass spectrometric analysis of plant lipids has been advanced accordingly. The branch of lipidomics in analysis of plant cellular lipids utilizing mass spectrometry was specifically called plant lipidomics [1], As described early in the book (Chapter 3), all kinds of MS-based lipidomics approaches, particularly those based on ESl-MS or ESI-MS/MS, could be used in plant lipidomics. The advantages of ESl-MS-based methods over traditional lipid analytical approaches for analysis of plant lipids were extensively discussed [1-3]. 

The developers concluded that LipidBlast could be successfully applied to analyze MS/MS data from over 40 different mass spectrometer types and used with other available search engines and scoring algorithms, which represents a paradigm shift in lipidomics because it is not feasible to chemically synthesize all metabolites or natural products as authentic standards for library generation or quantification purposes. Moreover, the current array of MS/MS mass spectra for plant, animal, viral, and bacterial lipids in LipidBlast could be readily extended to many other important lipid classes. 

DAG species for galactolipid biosynthesis. Then, the question is what causes the loss of GPL content under phosphorus deficiency conditions. Further studies by lipidomics [65, 66] revealed that the loss of PLDi, but not PLDi l, led to a significantly decreased accumulation of PA content in roots under phosphorus deficiency conditions and compromised the ability of plants to hydrolyze GPLs and to increase galactolipid levels. Moreover, plants null of PLD l and PLD( 2 exhibited shorter primary roots than wild-type plants under the phosphoras deficiency conditions. These results indicate that PLD l and PLDt play an important role in regulating root development in response to nutrient limitation. 

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