Piperine analysis

For the assay of piperine in pepper, a 25 m by 0.5 mm I.D. glass capillary deactivated by high temperature silanization and coated with 0V-1 was used. The samples were injected by the on-column injection technique at 100°C, as described by Grob and Grob Jr.. The standard deviation of the whole procedure, sample preparation and chromatographic analysis, was 2.5 %. 

GLASS CAPILLARY GC ANALYSIS WITH COLD ON-COLUMN INJECTION OF PIPERINE2 A = pure piperine and B = pepper extract. Internal standard (1) tetrahydropiperine, and piperine (2). Column 25 m x 0.5 m I.D. HTS-OV-1 column, 250°C isothermal. 

Gopu, C.L., Aher, S., Mehta, H., Paradkar, A.R., and Mahadik, K.R. 2008. Simultaneous determination of cinnamaldehyde, eugenol and piperine by HPTLC densitometric method. Phytochemical Analysis PCA, 19(2), 116-121. 

The analysis of Indian black pepper oil was reviewed [189] and the effect of peppercorn maturity on the piperine content of extract was reported [190]. The history, botany, chemistry, uses and markets for pepper have been published by several authors and well summarised in reference [191]. 

By sensory analysis is measured the sharpness (heat) of hot oieoresins, which is expressed in Scoviiie Heat Units. 50% of piperine. 

Analysis of the Natural Spice Ingredients Capsaicin, Piperine, Thymol and Cinnamaldehyde 1609... 

This application describes the analysis of extracts from spices using GC-MS/MS as a highly selective tool for the quantitative trace determination of the representative ingredients of natural active spice ingredients capsaicin, piperine, thymol, and also cinnamaldehyde as a flavouring side component. 

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