Pig adrenals

Fritz, J., Anderson, R., Fee, J., Palmer, G, Sands, R.H., Tsibris, J.C.M., Gunsalus, I.C., Orme-Johnson, W.H., and Beinert, H. 1971. The iron electron-nuclear double resonance (ENDOR) of two-iron ferredoxins from spinach, parsley, pig adrenal cortex and Pseudomonas putida. Biochimica et Biophysica Acta 253 110-133. 

Similar results to those of Nakajim et al. [82] have been obtained using guinea-pig adrenals [83], from which a cyt P-450 can catalyse both the 17-hydroxylation of progesterone and the SCC of 17-hydroxyprogesterone. 

Ferredoxins with (2Fe + 2S have also been reported in Aethusa, sica, Botrydiopsis, Bumilleriopsis, Chenopodium, Chlamydomonas, locasia Cyanidium, Cyperus, Datura, Equisetum, Euglena, Gossy-Phaseolus, Phormidium, Pinus, Pisum, Polystichum, Porphyrid-Zea, pig adrenals, pig testes. 

The yield of adrenodoxin from one kg of pig adrenal gland is approximately 10.0 mg of protein. An alternative method has been reported by Omura et al. (45, 46). 

Mitochondrial fractions from the adrenals of several species contain enzymes necessary for steroid production. These include a solubilizable Choi side chain splitting complex, a non-soluble cytochrome P-450, 18-hydroxylase and I8-0I dehydrogenase from sheeps and bullfrog adrenals and enzymes from the guinea pig adrenal which 11- and 21-hydroxylat reduce ring A and remove side chain of... 

SKF-1218. 2-(p-aminophenyl)-2-phenylethylamlne Inhibits B production in vitro by the rat and F production 1 vitro and to vivo by the guinea pig adrenal. Signs of adrenal insufficiency are produced in rats. Clinical studies suggest that like M, the drug is partially an 11- and 18-hydroxylase inhibitor 9, 50 ... 

Shinzawa, K., S. Kominami, and S. Takemori (1985). Studies on cytochrome P-450 (P-45017c<, lyase) from guinea pig adrenal microsomes. Dual function of a single enzyme and effect of cytochrome b. Biochim. Biophys. Acta 833, 151-160. 

Northern analysis of poly(A ) RNA from various tissues revealed that a 4.2 kb transcript is found predominantly in brain. 5-HTg mRNA is strongly expressed in olfactory tubercles, striatum and nucleus accumbens. Signals of moderate intensity were also found in the hippocampus (CAl, CA2, CA3 fields and dentate gyrus), olfactory bulb and cerebral cortex. In peripheral tissue a low expression was detected in rat stomach and guinea pig adrenals [12,13]. 

Guinea pig Adrenal Brain cortex Cerebral cortex Cerebral cortex... 

W.R. Dunham, et al., 2-Iron ferredoxins in spinach, parsley, pig adrenal cortex, Azotobacter vinelandii, and Clostridium pasteur-ianum—studies by magnetic field Mossbauer spectroscopy. 2, Biochim. Biophys. Acta 1971, 253(1), 134. 

The same diketopiperazine was recently isolated by Kuizenka et al. (360) from pig adrenal cortex. No physiological, hormonal, or other activity has been found for this peptide (636). 

The further transformation of (22S)-22-hydroxycholesterol studied with a guinea pig adrenal acetone-dried preparation was considerably smaller (under equivalent conditions) than that realized with the 22R isomer. Thus only 0.75% of pregnenolone was formed from the (22S) as compared to 16% formed from the (22R)-22-hydroxycho-lesterol. Similarly, the transformation of the (22S)-22-hydroxycho-lesterol to (22S)-20a,22-dihydroxycholesterol was insignificant as compared to the 20a-hydroxylation of the 22R isomer. 

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