Physiological Hints for the Production of Artificial Microbubbles

Accordingly, a detailed series of tests in this laboratory revealed that aqueous solutions of saturated monoglycerides (with acyl chain lengths greater than 10 carbons) combined with 

2 LASER-BASED FLOW CYTOMETRY AND FORWARD-ANGLE LIGHT SCATTERING 

All experimental measurements were carried out with a Coulter EPICS V System. This instrument is a laser-based flow cytometer which, as one of its simpler analytical functions, utilizes light scatter measurements to accurately size cells or similar particles (e.g., artificial surfactant-stabilized microbubbles) suspended in aqueous media. The light scatter measurements are sensitive to particle sizes as small as 0.3 pm in diameter. 

An estimate of the actual concentration of synthetic microbubbles present in the (shaken) artificial-microbubble-surfactant solution, represented by Fig. 9.1(A), is given by the fact that 360-400 particles/sec were consistently detected at a flow rate of approximately 1 ml/30 min in order to produce the histogram shown. Therefore, the calculated approximate concentration of synthetic microbubbles in the sample is 7 x 105 microbubbles/ml. (A similar calculation for the distilled water sample shown in Fig. 9.1(B) results in an estimated concentration of only 5 x 103 microbubbles/ml.) 

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