Physarium

The nuclei of the acellular, slime mold Physarium polycephalum contains a /3-D-gaIactan (d.p. 560) bearing phosphate (2.5%) and sulfate (9.6%) groups. One unit in every 13 is branched, but the main structural-feature is 4-O-substituted D-galactopyranosyl units.121 It resembles the exocellular polysaccharide.122... 

The myxomycete Physarium polycephalum produces hard-walled sclerotia (spherules) that contain 88% of 2-amino-2-deoxy-D-galactose. The positive, specific rotation, and the g.l.c. characterization of 1,5-di-... 

PolyPs with an average chain length of 100 residues were observed in the nuclei of Physarium polycephalum (Pilatus et al, 1989). Nuclear preparations of mammalian cells were found to be relatively enriched in PolyPs (Kumble and Kornberg, 1995 Kornberg, 1999). 

A17. Atmor, V. J., and Keuhn, G. D., Effect of polyamines in adenyl cyclase activity and acidic nuclear protein phosphorylation in physarium polydephalum. Fed. Proc. Fed. Am. Soc. Exp. Biol. 36, 686 (1977). 

Solnica-Krezel L, Burland TG, Dove WF. Variable pathways for developmental changes of mitosis and cytokines in Physarium polycephalum. J Cell Biol. 1991 113 591-604. 

RNA. A method analogous to that for DNA" has been applied to RNA, the 5 -terminally-labelled RNA being partially hydrolysed by alkali or base specific ribonucleases. The scope of this method has been extended by use of the non-specific endo-nuclease Pi and the ribonuclease from Physarium poiy cepharum which cleaves all but CpN linkages, to distinguish between oligonucleotides with Up or Cp 3 termini generated by RNase A from pancreas or RNase from Bacillus cereus. ... 

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