Photodynamic treatment virus inactivation

C.D. Lytle, P.G. Carney, R.P. Felten, H.F. Bushar, R.C. Straight (1989). Inactivation and mutagenesis of herpes virus by photodynamic treatment with therapeutic dyes. Photochem. PhotobioL, 50(3), 367-371. 

Currently, all donors and blood preparations undergo multistage and expensive control to ensure the absence of viral contamination In this respect, the development of affordable methods of inactivation of viruses could be an important step toward safety in hemotransfusion. Currently used treatments such as UV irradiation damage therapeutic components of the blood (Williamson and Cardigan, 2003), so alternative selective approaches are needed for this purpose. Among them, chemotherapy, photochemotherapy (PCT), and photodynamic antibacterial therapy should be noted (Mohr, 2000). 

Recently, it has been shown that photoactive fullerene derivatives (Cgo) were very effective in the photodynamic inactivation of bacterial viruses (bacteriophages). The treatment of water with CgQ-based PS under illumination resulted in a 2-log reduction in the number of bacteriophages in the water within only 2 min (Lee et ah, 2009). 

Photodynamic ACT (PACT), similar to photodynamic therapy (PDT) described in Chapter 17, utilizes photosensitizers and visible or UV light in order to induce a phototoxic response, usually via oxidative damage. For some time the disinfection of blood products, particularly for viral inactivation, has been the major use of PACT, although more and more clinically based protocols are being developed, eg in the treatment of oral infections. The technique has been shown to be effective in vitro against bacteria (including drug resistance strains), yeasts, viruses, and parasites. 

R.H. Kaufman, E. Adam, R.R. Mirkovic, J.L. Melnick, R.L. Young (1978). Treatment of genital herpes simplex virus infection with photodynamic inactivation. Am. J. Obstet. Gynecol, 132(8), 861-869. 

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