Photochemical Control by Enzyme-bound Photoisomerizable Units

Photochemical Control by Enzyme-bound Photoisomerizable Units 

The enzyme GOx was transformed into a photoswitchable enzyme by its chemical modification with nitrospiropy-ran photoisomerizable units [382]. The 

To optimize the photoswitchable bioelectrocatalytic features of redox enzymes, the site-specific functionalization or mutation of the active-site microenvironment is essential. This has been accomplished by a semisynthetic approach, involving the reconstitution of the flavoen-zyme GOx with a semisynthetic photoisomerizable FAD-cofactor [383, 384]. Nitrospiropyran carboxylic acid (27) was covalently coupled to iV -(2-aminoethyl)-FAD (19) to yield a photoisomerizable nitrospiropyran-FAD cofactor (28a) 

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