Phosphylated nonapeptide

Blood Analysis of phospho-rvlated peptides Phosphyla-ted BuChE LC-MS-MS (after enzymatic digestion of modified cholinesterase. Phosphylated nonapeptides i.s. plasma exposed to CD3-OP Covers all OP s LOD 1-5% BuChE inhibition Expensive instrumentation and reference compounds... 

A major drawback of the fluoride reactivation method is that not all nerve agent adducts are amenable to fluoride reactivation, with the aged adduct of soman the best known example. This problem can be solved by looking at the BuChE enzyme itself Fidder et al (2002) published a method based on the LC-MS analysis of a nerve agent phosphylated nonapeptide derived after pepsin digestion of inhibited butyrylcholinesterase. The authors presented a procedure to extract BuChE from plasma using... 

Van der Schans et al., 2002). It is based on the rapid isolation of BChE from plasma by affinity chromatography, digestion with pepsin, and followed by liquid chromatography with toe mass spectrometric analysis of phosphylated nonapeptides. The method can be applied for the detection of exposures to various OP pesticides and nerve agents including soman. This approach is very valuable and represents a new field for the improvement of diagnosis with OPs. A comprehensive review of the methods for retrospective detection of exposure to toxic scheduled chemicals has been published by Noort et al. (2001, 2002). 

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