Phosphotungstic acid hematoxylin

Figure 2. Photomicrographs (a) and (b) show low and high power views, respectively of a phosphotungstic acid hematoxylin-stained 5 yum thick section of lung from a mouse given a lethal dose of toxin-LR, and then killed when moribund. The arrow in (a) points to a thrombus in a pulmonary artery. The same thrombus is shown at higher magnification in (b). The thrombus consists of irregular clusters of small granules.

The hbrillary astrocytoma is more common than the protoplasmic astrocytoma. " Fibrillary astrocytomas are a mixture of cellular processes (fibrils) and nuclei of greater angularity and density than normal or reactive astrocytes (Fig. 20.13 see Fig. 20.1 IB). They contain more intracytoplasmic fibrils, and their cellular processes are longer than those in protoplasmic astrocytomas. Thus, only the fibrillary astrocytoma stains well with phosphotungstic acid hematoxylin (PTAH), which stains fibrillar protein arrays, whereas both... 

Fig. 31-2. Lung of a rhesus monkey that died of inhaled staphylococcal enterotoxin B (SEB) intoxication, (a) Intraalveolar fibrin deposition and hyaline membrane formation can be seen (hematoxylin-eosin stain, original magnification x 50). (b) Intraalveolar deposition of polymerized fibrin can be seen (phosphotungstic acid hematoxylin stain, original magnification X 50).

Fig. 32-4. Lungs from a monkey exposed to ricin by aerosol, (a) The lungs are edematous, with accompanying hemorrhage and necrosis, (b) Histologically, the microscopical changes are characterized by fibrinopurulent pneumonia. The fibrin has been specifically stained by phosphotungstic acid hematoxylin to appear purple (original magnification x 25). Photographs Courtesy of CL Wilhelmsen, DVM, PhD, Lieutenant Colonel, Veterinary Corps, US Army Division of Pathology, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Md.

For light microscopic examination, liver tissue was fixed in 10 % buffered formalin, embedded in paraffin, sectioned and stained with hematoxylin and eosin. In some cases, preparations were stained with PTAH (phosphotungstic acid-haematoxylin), by the Van Gieson method and the PAS (periodic acid-Schiff)... 

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