Phosphor imaging analyzer

Bioimaging/Phosphor Imaging Analyzers (Fuji and Molecular Dynamics)... 

Rf values, 4,7,931-934 Radiation detectors, 349-355 bioimaging/phosphor imaging analyzers, 354-355,357... 

Figure 3. Membrane skeletal association of FPR. Neutrophils were solubilized with Triton X-100 and the extracts were spun over sucrose density gradients. The distribution of photoaffinity labeled FPR was analyzed on SDS-polyacrylamide gels with a Phosphor Imager. Percent receptors that are coupled to the membrane skeleton are shown for responsive control cells (C), partially (PD) and fully (FD) desensitized neutrophils. In addition, values are shown for cells after solubilization in the presence of agents causing depolymerizaiton of actin filaments, like KCl, p-chloromercuriphenylsulfonic acid (pCMPS) or DNAse I. For experimental details see [44].

Comparisons of the various TLRC methods are summarized in Tables 13.1 (Shul-man, 1983 Shulman and Weaner, 1991) and 13.2 (Clark and Klein, 1996). Linear analyzers are still widely used in many laboratories because they offer reasonable speed, resolution, sensitivity and quantitative accuracy that is adequate for many applications and are less expensive than the newer detection instruments. When the highest level of sensitivity, quantification, and resolution are required, use of a multiwire proportional counter or phosphor imager is in order despite their high price. The field appears to be moving rather quickly toward much wider use of phosphor imagers, and it is likely this trend will continue at the expense of proportional counters. 

Parameters Autoradiography Zonal analysis Linear analyzer MWPC detector Phosphor imager... 

There are three principal techniques for the analysis of radioactive components on TLC plates, autoradiography, zonal analysis, and mechanical detectors (e.g. linear analyzers, phosphor imagers, MWPC detectors). The technique of choice depends on a number of parameters but of primary consideration are sensitivity and resolution. Other parameters that are to be considered are quantitation, linear dynamic range, speed, sample throughput, and preservation of the sample. A comparative sununary of the detection methods with respect to these parameters is shown in Table 2. 

Both autoradiography and zonal analysis have a number of drawbacks, including sensitivity and resolution, but primarily both techniques are extremely time consuming. Linear analyzers offer a good compromise among speed, resolution, and quantitative accuracy. However, the performance of the linear analyzers falls well below that of the currently available MWPC detectors and phosphor imagers in all respects. Sensitivity, quantitation, and particularly resolution are significantly superior... 

Figure 3.1 schematically represents time-resolved experimental setup used in our experiments. The excitation sources were pulsed lasers, such as excimer XeCl (308 nm), nitrogen (337 run), three harmonics of Nd-YAG (266, 355 and 532 nm), and tunable dye and OPO, which deliver pulses of 10 ns duration. The spectra observed at the geometry of 90° are analyzed by intensified CCD matrix. Image intensifiers comprise three main components a photocathode, microchannel plate (MCP) and phosphor screen. The standard operation of these devices starts when the incident photons become converted into electrons at the photocathode. The electrons then accelerated towards the MCP where they are multiplied to an amount... 

Fig. 7 X-ray photoelectron spectrometer. Left schematic view of a SSX 100/206 (Surface Science Instruments). Right, photographs of a Kratos Axis Ultra (Kratos Analytical) with the introduction and intermediate chambers (top) and analysis chamber (bottom), a, Turbomolecular pump b, cryogenic pump c, introduction chamber d, sample analysis chamber (SAC) e, transfer probe f, automatized X, Y, Z manipulator g, X-ray monochromator h, electrostatic lens i, hemispherical analyzer (HSA) j, ion gun k, aluminum anode (with monochromator) 1, aluminum-magnesium twin anode m, detector. Left channel plate. Right 8 channeltrons (Spectroscopy mode), phosphor screen behind a channel plate with a video camera (Imaging mode) n, spherical mirror analyzer (SMA) o, parking facility in the sample transfer chamber p, sample cooling device for the introduction chamber q, sample transfer chamber r, monitor interconnected with the video camera viewing samples in the SAC s, video camera in the SAC t, high temperature gas ceU (catalyst pretreatment)...

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