Phosphomolibdic acid

Coomassie Brilliant Bine Phosphomolibdic acid Antimonytrichloride... 

A) Reference mixture of simple lipid classes, silica gel TLC, mobile phase hexane-ethyl ether-acetic acid, 80 20 2 (v/v/v) SE, sterol esters ME, fatty acid methyl esters TG, triacylglycerols EEA, free fatty acids S, sterols 1,3-DG, 1,3-diacylgly-cerols 1,2-DG, 1,2-diacylglycerols MG, monoacylglycerols C, complex lipids detection by spraying with 5% ethanolic phosphomolibdic acid and heating for several minutes at 180°C. 

The silver nitrate-impregnated silica gel G was used to obtain a separation with a group of tetracyclic triterpene alcohols, which could not be separated on untreated silica gel. However, on silica gel G (Ag impregnated), the R values were sufficiently different so as to enable the substances to be identified separately. Chloroform was used as the mobile phase. The tetracyclic triterpenes were detected by spraying with one of the three reagents 50% sulfuric acid in water or 10% phosphomolibdic acid in ethanol or chlorosulfonic and acetic acids (1 2) followed by heating in an oven at 150°C. ... 

Evaluation reagents Col-1 ethanolic or methanolic sulfuric acid-l-UV254, Col-2 alkaline tetrazolium blue. Col 3 antimony/III/chloride, Col-4 perchloric acid, Col-5 p-toluene sulfonic acid, Col-6 chloramine T, Col-7 arzenomolybdate, Col-8 phosphomolibdic acid. 

Evaluation reagents Coi-I ethanolic or methanoiic sulfuric acid -t L V365, Col-2 alkaline tetrazoiium blue, Coi-3 phosphomolibdic acid. Col 4 antimony/Ill/chloride, Col-5 zinc chloride, Col-6 silver nitrate. 

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