Phosphoenol pyruvate synthetase

Some of the reactions of PO3- parallel enzymatic reactions promoted by adenosine triphosphate (ATP). Pyruvate kinase catalyzes the equilibration of ATP and pyruvate with adenosine diphosphate (ADP) and phosphoenol pyruvate (11,12). In a formal sense, this reaction resembles the preparations of enol phosphate (eqs. 6 and 7). Cytidine triphosphate synthetase catalyzes the reaction of uridine triphosphate with ammonia to yield cytidine triphosphate (13). In a formal sense, this reaction resembles the replacement of the ester carbonyl group of ethyl acetate by the nitrogen of aniline (eq. 8). 

AcK acetate kinase AcP acetyl phosphate AdK adenylate kinase AP A p ,pn-di(adenosine 5 -) n-phosphate ARS aminoacyl tRNA synthetase ATP, ADP, AMP adenosine 5 -tri-, di-, monophosphate ATP-u-S (Sp)-adenosine 5 -0-(l-thiotriphos-phate), ATP-y-S adenosine 5 -0-(3-thiotriphosphate) CK carbamyl kinase CP carbamyl phosphate CrK creatine kinase CTP, CDP, CMP cytidine 5 -tri-, di-, monophosphate dATP, dAMP deoxyadenosine 5 -tri-, monophosphate DNA deoxyribonucleic acid AG change in free energy GK glycerol kinase GTP, GDP, GMP guanosine 5 -tri-, di-, monophosphate HK hexokinase IUB International Union of Biochemistry MCP methoxycarbonyl phosphate NTP, NDP, NMP nucleoside 5 -tri-, di-, monophosphate PC phosphocreatine PEP phosphoenol pyruvate P orthophosphate PK pyruvate kinase P polyphosphate PnK poly-... 

KDO synthetase catalyzes the reaction of arabinose 5-phosphate (29 Ara-5-P) and phosphoenol pyruvate (PEP) to form KDO-8-P (30 Scheme 9). KDO synthetase is not commercially available but has been isolated from E. coli and used in the synthesis of KDO-8-P (63% from Ara-5-P, 38 mmol). KDO-8-P is a key intermediate in the synthesis of the lipopolysaccharide region of Gram-negative bacteria (LPS). Inhibitors of LPS biosynthesis are targets for the design of antimicrobial drugs. ... 

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