Phosphatidylinositols fluorescence

Lipid-protein interactions are of major importance in the structural and dynamic properties of biological membranes. Fluorescent probes can provide much information on these interactions. For example, van Paridon et al.a) used a synthetic derivative of phosphatidylinositol (PI) with a ris-parinaric acid (see formula in Figure 8.4) covalently linked on the sn-2 position for probing phospholipid vesicles and biological membranes. The emission anisotropy decays of this 2-parinaroyl-phosphatidylinositol (PPI) probe incorporated into vesicles consisting of phosphatidylcholine (PC) (with a fraction of 5 mol % of PI) and into acetylcholine receptor rich membranes from Torpedo marmorata are shown in Figure B8.3.1. 

Phosphatidylinositol containing fluorescent fatty acids has also been prepared [303] by synthesising a phosphatidylcholine containing fluorescent fatty acids and then using a phospholipase D, in the presence of inositol, to effect an ester interchange. 

Holz RW, Hlubek MD, Sorensen SD et al (2000) A pleckstrin homology domain specific for phosphatidylinositol 4, 5-bisphosphate (PtdIns-4,5-P2) and fused to green fluorescent protein identifies plasma membrane PtdIns-4,5-P2 as being important in exocytosis. J Biol Chem 275 17878-85... 

One of the metabolic enzymes for Ins(1,4,5)P3, Ins(1,4,5)P3 3-kinase isolated from rat brain cortex was used to prepare Ins(1,3,4,5)P4 from Ins(1,4,5)P3.88 myo-Inositol (1) was converted directly without protection to fluorescent 1-phosphatidylinositol... 

The preparation of a photoaffinity probe possessing biotinyl and azidobenzoyl moieties of Ins(l,4,5)P3 has been described which has similar biological function and potency to IP3 with respect to inhibition of [ H]IP3-5-phosphatase as well as the ability to photolyse at the IP3 recognizing domain of the protein. The synthesis of the myoinositol derivative (84) as a fluorescent substrate for assaying phosphatidylinositol-specific PLC has been reported. ... 

MQAE Ar-Ethoxycarbonylmethyl-6-methoxyquinolinium bromide NADH Reduced nicotinamide dinucleotide PBFI Potassium-binding fluorescence indicator PI-TP Phosphatidylinositol-transfer protein PKC Protein kinase C... 

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