Phosphate protein hydrolysates, high

Infants treated before 1979 received protein hydrolysates containing high concentrations of phosphate which limited the concentration of calcium that could be used without causing precipitation. Beginning in 1979 crystalline amino acid solutions which contained less obligatory phosphate became available these allowed greater latitude in the concentrations of calcium and phosphate that could be achieved. The data in Table I suggest that the severity of demineralization and the incidence of fractures and rickets will decrease if more calcium is added to the parenteral alimentation solution. 

High Performance Liquid Chromatography.—This technique has been applied to a wide range of natural products, i.e., steroidal phosphates, retinyl phosphate, dansylated protein hydrolysates, thiamine phosphates, dibutyl c-AMP esters, cytosine arabinoside triphosphate, and di- and tri-nucleoside phosphates. ... 

Treatment with hot organic solvents was the next step in the tissue fractionation, to remove lipid-phosphorous and breakdown lipid-protein interactions. In the Schneider procedure, nucleic acids were then extracted in hot dilute trichloroacetic or perchloric acid, leaving a protein residue with any phosphoprotein links still intact. This method was to become particularly useful when 3H thymidine became the preferred label for DNA in the early 1960s. For investigations where both RNA and DNA were to be examined the Schmidt-Thannhauser process was often chosen. Here the lipid-extracted material was hydrolyzed with dilute sodium hydroxide releasing RNA nucleotides and any hydroxyamino acid bound phosphorus. DNA could be precipitated from the extract but the presence in the alkaline hydrolysate of the highly labeled phosphate released from phosphoprotein complicated... 

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