Phosphate-buffered saline/ tween PBST

Enzyme-linked immunosorbent assay (ELISA). Li and Li developed an ELISA procedure for imidacloprid to determine its residues in coffee cherry and bean extracts. A 25-g amount of sample extracted with 300 mL of methanol and 1% sulfuric acid (3 1, v/v) for 3 min. An aliquot of the sample extract (0.5 mL) is mixed with 1 mL of water and a gentle stream of nitrogen is used to evaporate methanol. The solution is then extracted with 1 mL of ethyl acetate, the extract is reconstituted in 1 mL of PBST (phosphate-buffered saline containing 0.05% Tween 20) and competitive ELISA is performed to quantify imidacloprid in the extract. Eor methanol extracts of coffee cherries and beans fortified with imidacloprid at 0.5 mgL recoveries of imidacloprid by the ELISA method were 108 and 94, respectively. 

PBST Phosphate-buffered saline with Tween-20... 

Table 8 Inhibition of FMAP in f-BSA Microspheres after 60-Day Release in Phosphate Buffered Saline Containing 0.02% Tween 80 (PBST) at 37°C...

Polyethyleneimine, 1% solution in water PBS (phosphate-buffered saline), lOx stock PBST (PBS + 0.1% Tween-20)... 

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