Phosphate adenosine sulfatophosphate

Inorganic sulfate is transformed to adenosine-5 -sulfatophosphate (ASP) and 3 -phosphate adenosine-5 -sulfatophosphate (PAPS, activated sulfate . Fig. 186) in microorganisms, plants, and animals. The sulfate group of ASP and PAPS may be reduced to the level of sulfide in microorganisms and plants. Carrier-bound sulfide reacts with 0-acetyl-L-serine or L-serine (D 10) to L-cysteine. 

Baddiley et at. used the reagent to convert adenosine 3, 5 -diphosphate into adenosine 3 -phosphate-5 -sulfatophosphate. In the presence of an appropriate enzyme, this active sulfate transfers the sulfate group to a variety of substrates. 

Conjugation of 0-, N- and S-contalnlng functional groups with sulfate Is very common In animal systems. Sulfate Is transferred from adenosine 3 -phosphate-5 -sulfatophosphate (PAPS) by a variety of transfer enzymes. The mechanisms for sulfation of xenobiotics (74,75) and the chemistry of sulfate esters and related compounds and the synthesis of these have been reviewed (75-77). Syntheses of 0-, N- and S-sulfate conjugates of xenobiotics may be carried out by the methods described below. 

The method established has been applied in particular to the assessment of the effects of L-ascorbic add, vitamin A, triparanol, and dimethyl sulfoxide on human skin (B9), on account of uses of these compounds in therapy. The effect of triparanol on incorporation of [ S]sulfate into proteoglycans and adenosine 3 -phosphate 5 -sulfatophosphate of the epidermis was reduction in each case, but an increase into those of the dermis, thus demonstrating a serious disruptive effect of the drug on tissue processes. 

Schwenn J D, Jender H G 1980 Reversed-phase high-performance liquid chromatography of adenine nucleotides application to the kinetics of an adenosine 3 -phosphate 5 -sulfatophosphate sulfotransferase from plants. J Chromatogr 193 285-290... 

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