Pheochromocytoma cells catecholamine release from

Release of Catecholamines from the Adrenal Medulla Angll stimulates catecholamine release from the adrenal medulla by depolarizing chromaffin cells. Although this response usually is of minimal physiological importance, dangerous reactions have followed Angll administration to individuals with pheochromocytoma. 

Taylor SC, Shaw SM, Peers C. Mitochondrial inhibitors evoke catecholamine release from pheochromocytoma cells. Biochem Biophys Res Commun 2000 ... 

Chen, T.K., G.A. Luo, and A.G. Ewing. 1994. Amperometric monitoring of stimulated catecholamine release from rat pheochromocytoma (PC12) cells at the zeptomole level. Anal. Ghent. 66 3031-3035. 

Recent studies have shown that cyanide releases catecholamines from rat pheochromocytoma cells and brain slices (Kanthasamy et al. 1991b), from isolated bovine adrenal glands (Borowitz et al. 1988), and from the adrenals of mice following subcutaneous injection of high doses of potassium cyanide (Kanthasamy et al. 1991b). Thus, it was proposed that the cardiac and peripheral autonomic responses to cyanide are partially mediated by an elevation of plasma catecholamines (Kanthasamy et al. 1991b). 

Permeabilized secretory cells are widely used to study the final events during secretion by exocytosis. Convenient cellular models are bovine adrenal chromaffin cells in short term culture and the rat pheochromocytoma cell line PC 12. Both cell types take up labeled catecholamines and store them in secretory vesicles, from which they can be released upon stimulation. The released catecholamines can be detected in the supernatant. After permeabilization of the plasma membrane, release of catecholamines can be triggered by micromolar concentrations of Ca. ... 

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