Phenylephrine. HPLC

Phenylephrine (27) is a low-potency sympathomimetic amine used as a decongestant. Solutions become coloured due to an auto-oxidation accelerated by light. In a series of experiments, aqueous solutions of the hydrochloride were left under a UV lamp until a tan colour developed. HPLC analysis showed four main products of which one was identified as adrenaline (19). Even after prolonged irradiation, there was never more than 2% adrenaline in the solution. It was assumed that the catecholamine was removed as it formed by further reaction to adrenochrome and melanine, which accounted for the colour [34],... 

G. Parhizkani, R. B. Miller, and C. Chen, A stability-indicating HPLC method for the determination of benzalkonium chloride in phenylephrine HG 10% ophthalmic solution, J. Liquid Chromatogr., 78 553 (1995). 

Antipyrine Bulk HPLC Silica CH2Cl2-MeOH (1% NH4OH) (98 2) 254 nm GC [9] ear drops [153,154] USP 23, ordinary impurities, p. 142, with benzocaine solution. with phenylephrine, assay by HPLC, p. 125 [5] [9]... 

HPLC HPLC Dissolve in water Dilute with water C-18 C-18 Buffer-water (7 3), pH 3.4 Buffer-0.007M DCS-0 007M ammonium nitrate 280 nm USP 23, p. 482 resolved from bamipine and phenylephrine [424] [5]... 

Achari, R.G. Jacob, J.T. A study of the retention behavior of some basic drug substances by ion-pair HPLC. J.Liq.Chromatogr., 1980, 3, 81-92 [also N-acetylprocainamide, antazoline, caffeine, chlorpheniramine, codeine, ephedrine, epinephrine, naphazoline, papaverine, pheniramine, phenylephrine, phenylpropanolamine, procainamide, quinidine, scopolamine, xylocaine]... 

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