Phenyl-ammonia lyase route

L-Phe can be prepared via the enantioselective hydrolysis of N-acetyl-D,L-Phe and microbial reductive amination of phenylpyruvate (see Fig. 8.14) [87]. The stoichiometric yields of these processes were high but the precursors required 3-4 synthetic steps from the basic starting materials in most cases. The phenyl-ammonia lyase route, in contrast [89], provided L-Phe in only two steps from the basic chemicals benzaldehyde and acetic anhydride [90]. The enzymatic step... 

Aspartic acid, alanine, phenylalanine, and lysine were manufactured by enzymatic route. Immobilized E. coli cells expressing aspartate or the immobilized enzyme has been used in the commercial production of aspartic acid from ammonia and fumaric acid. Chibata and coworkers also produced alanine by microbial Pseudomonas dacunhae) L-aspartate P-decarboxylase with aspartate as the starting material. Phenyl alanine was manufactured from fw s-cinnamic acid and ammonia by the enzymatic route by phenyl alanine ammonia lyase as catalyst or from phenyl pyruvate and aspartic acid using transaminase. 

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