Phenomenex

Proteias, amino acids bonded through peptide linkages to form macromolecular biopolymers, used as chiral stationary phases for hplc iaclude bovine and human semm albumin, a -acid glycoproteia, ovomucoid, avidin, and ceUobiohydrolase. The bovine semm albumin column is marketed under the name Resolvosil and can be obtained from Phenomenex. The human semm albumin column can be obtained from Alltech Associates, Advanced Separation Technologies, Inc., and J. T. Baker. The a -acid glycoproteia and ceUobiohydrolase can be obtained from Advanced Separation Technologies, Inc. or J. T. Baker, Inc. 

With large companies such as Waters Corp., TOSOH Corp., Polymer Laboratories Ltd., Shodex Corp., and Phenomenex Inc. and smaller companies such as American Polymer Standards Corp., Polymer Standards Service, and Jordi Associates, all manufacturing ST-DVB GPC/SEC gel, the market has become saturated with analogous columns. All the manufacturers just men-... 

Phenomenex, Columbus Cg, 50 mm x 2.0-mm i.d., 5-p.m particle size, 100-A pore size Notes l. Other brands tested did not retain RPA 202248. 

Tokyo, Japan) Phenomenex Chirex 3019 S-ferf-Leu and Pirkle type... 

Polystyrene THF, toluene 162-2 x 107 Narrow Phenomenex , Waters, Polymer Laboratories... 

The reaction mixture is sampled after each reaction cycle and analyzed for purity, reaction progress, and the presence and amount of by-products (if any) formed. Analysis is by gas chromatography (HP 5890 Series II Phenomenex Zebron ZB-1 capillary column, Phenomenex Cat. No. 7HK-G001-36) with flame ionization detection in order to quantify the by-product impurities. The quantity of the byproducts is determined using an external standard calibration method. 

A Waters 2690 Alliance HPLC equipped with a 996 photodiode array and a 896 IJV/Vis detector was used for carotenoid analysis. The column (Phenomenex, Torrance, CA) was a 250x4.6mm Ultracarb 3 pm C-18 stationary phase and elution was carried out isocratically at a flowrate of l.OmL/min with 85 15 (v v) acetonitrileimethanol (HPLC grade) containing 0.1% triethyl amine to prevent on-column carotenoid decomposition. 

Figures 10 and 11 show the structure of the hindered phenolic antioxidant Irganox 1010 (Ciba) and its negative ion APCI mass spectra, respectively. Separation was achieved under the following LC conditions Column Aqua Cl 8 (Phenomenex) 3 pm, 150x2.00 mm, 15% carbon loading, proprietary end capping. Column Temp 50°C. Injection volume 5 pi.

The related synthetic antistat Atmer 163 can be detected and quantified in a similar manner. Extraction from samples using hexane/isopropanol (IPA) 30/70 mixtures is employed. Figure 14 shows separation using an Agilent Ion-Trap SL LC-MS with a Phenomenex Aqua Q8 column (150 x2 mm) at 45°C and 0.5ml/min. Solvent A was 0.5% formic acid in water and solvent B was acetonitrile. The solvent gradient was taken from 45% B to 70% B over 15 min and held for 5 min. The mass spectrometer was set to scan 280 to 300 m/z, targeting 288 m/z with ESI ionisation. 

Glycerol monostearate can also be determined by LC-MS. Detection by positive APCI is possible. Figure 15 shows a typical chromatogram for the technical product eluting from a Phenomenex Aqua Ci8 column with methanol-water gradient (70-100% methanol). The molecular ion (M + H)+ mass spectra of the two main peaks detected are shown in Figures 16 and 17. 

Rainbow 5 pm Phenomenex Gradient elution Diode-array [77]... 

Phenomenex (see 2006 Catalog, SPE products) Strata-X Polar functionalized styrene-divinylbenzene polymer Reversed phase with weakly acidic, hydrogen bond donor, acceptor, and dipolar interactions Cetirizine (76) pyridoxine (77) omeprazole (78) mycophenolic acid (79) 25-hydroxy-vitamin D3 (80)... 

Phenomenex Strata- Sulfonated styrene- Mixed mode with both strong Stanazolol (89) ... 

The complexity of the method in terms of number of steps and solvents needed depends on the sorbent chemistry. The development in a simplified scenario involves running an analyte in several concentrations in multiple replicates and assaying for recovery and performance. This procedure is described in detail for several silica and polymeric sorbents by Wells.42 However, if a number of sorbents are to be evaluated, the process becomes time-consuming if multiple 96-well plates (each with one sorbent packed in all the wells) must be screened separately. This process may take a week or more and consume an analyst s precious time as well. The most plausible solution is to pack different sorbents in the same well plate and use a universal procedure that applies to all of them. An example of such a multisorbent method development plate is the four-sorbent plate recently introduced by Phenomenex demonstrated124 to require only 1 to 2 hr to determine optimal sorbent and SPE conditions. 

FIGURE 1.18 Phenomenex four-sorbent SPE method development plate. 

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