Phenolic compounds flow injection systems

H.M. Oliveira, M.A. Segundo, S. Reis, J.L.F.C. Lima, Multi-syringe flow injection system with in-line preconcentration for the determination of total phenolic compounds, Microchim. Acta 150 (2005) 187-196. 

Analysis of Phenolic Compounds. A Hewlett-Packard (Palo Alto, CA) Model 1090 HPLC System, was used to determine the levels of specific phenolic components. The HPLC system was equipped with a ternary solvent delivery system, a diode array UV-VIS detector, and HP ChemStation software for data collection and analysis. Full chromatographic traces were collected at 280, 520, 316, and 365 nm, and spectra were collected on peaks. The stationary phase was a Hewlett-Packard LiChrosphere C-18 coliram, 4mm X 250 mm, with 5 pM particle size packing. Operating conditions include an oven temperature of 40 C, injection volume of 25 pL, and flow rate of 0.5 mL/minute. The mefriod was based on a previously published method for phenolic components in wine (30) and used the modified solvent gradient shown in Table II. Solvent A was 50 mM dihydrogen ammonium phosphate, adjusted to pH 2.6 with orthophosphoric acid. Solvent... 

Leamsomrong, K., M. Suttajit, and P. Chantiratikul. 2009. Flow injection analysis system for the determination of total phenolic compounds by using Folin-Ciocalteu assay. Asian J. Appl. Sci. 2(2) 184-190. 

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