Pharmacokinetic proteomics analysis

Pharmacokinetic proteomics (ADME analysis, formulation, stability studies, and pharmacokinetics). 

There are two main reasons for entering the realm of micro- and nano-LC to obtain necessary sensitivity or if only the smallest quantities of samples are available. The main fields of application are currently proteomics, pharmacokinetics, metabolism studies, microdialysis, and, increasingly, environmental analysis. Flow rates in micro- (2-50 pL min ) and nano-LC (200-2000 nL min ) place high demands on the HPLC system and the user. Continuous optimization with regard to robustness, sensitivity, detection limit, and resolution tends to be a feature of any application. 

Part V targets applications that are organized according to specific compound classes of analytes. The role of mass spectrometry in peptide and protein characterization and in proteomics is the subject of Chapter 18. Next, the topic of carbohydrate analysis by ESI and MALDI is tackled in Chapter 19. This is followed by an examination of ESI and MALDI applications to lipid analysis (Chapter 20). Finally, the important subject of drug discovery is addressed in Chapter 21, including in vitro ADME (absorption, distribution, metabolism and excretion) profiling and pharmacokinetic screening. 

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