Pharmacokinetic assays

LC/MS is the ultimate analytical technique, which combines the versatility of HPLC with the identification power of MS. The weak link in LC/MS has always been the interface which connects the liquid stream at atmospheric pressure to the high vacuum present inside the mass spectrometer. The development of several atmospheric pressure interfaces, electrospray and atmospheric pressure chemical ionization (APCI), has contributed to the tremendous success and popularity of LC/MS and LC/MS/MS in bioresearch, drug discovery, combinatorial analysis and pharmacokinetic assays. This topic is covered in more depth in a later chapter. 

Barbizhayev, M. A. (2005). Analysis of lipid peroxidation and electron microscopic survey of maturation stages during human cataractogenesis Pharmacokinetic assay of Can-C N-acetylcamosine prodrug lubricant eye drops for cataract prevention. Drugs 6, 345-369. 

The difficulties in conducting these pharmacokinetic assays - collecting excrement for relatively long period of time (24 h +), requirements for specialized cages and specialized protective apparatus, and the increased space requirements for housing animals individually -has led to the use of other in vivo assays and to the development of in vitro models. 

Drug Discovery and Evaluation Safety and Pharmacokinetic Assays... 

L.R. Brown, Biomaterials in their role in creating new approaches for the delivery of drugs, proteins, nucleic acids, and mammalian cells. Drug Discov. Eval. Saf. Pharmacokinetic Assays (2013) 677-690. 

Amouzadeh HR, Vargas HM (2013) Safety pharmacology assessment of biopharmaceuticals. In Vogel HG et al (eds) Drag discovery and evaluation safety and pharmacokinetic assays. Springer, Berlin, pp 555-560... 

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