Pharmaceuticals, separation system

Jia et al. (2005) developed a two-dimensional (2-D) separation system of coupling chromatography to electrophoresis for profiling Escherichia coli metabolites. Capillary EC with a monolithic silica-octadecyl silica column (500 x 0.2 mm ID) was used as the first dimension, from which the effluent fractions were further analyzed by CE acting as the second dimension. Multi-dimensional separations have found wide applications in biomedical and pharmaceutical analysis. 

In recent work within our laboratories, we have evaluated a number of unbonded silicas by CEC specifically for the separation of a range of pharmaceutically relevant basic analytes and mixtures. We purposefully chose strong basic analytes that comprised a wide range of lipophilicities, molecular weights and log P values to robustly test the separation systems. The basic analyte test mixture contained two AstraZeneca R D compounds, benzylamine, nortriptyline, diphenhydramine and procainamide. 

Heller, M.C., Carpenter, J.P., and Randolph, T.W., Conformational stability of lyophilized PEGylated proteins in a phase-separating system. Journal of Pharmaceutical Sciences, 88, 58, 1999. 

The importance to use optically pure isomers as pharmaceuticals, food additives, agrochemicals, (etc) is becoming more and more evident. The classical resolution still accounts for a large part of chiral production, however the asymmetric synthesis and the use of chiral separation system one becoming increasingly popular. The enantioseletive hydrolytic resolution of racemic epoxides was performed in the ZSM-5/MCM-41 membrane system containing chiral salen complexes. The chiral salen complexes immobilized on the membrane showed a very high enantioselectivity in the hydrolysis of epichlorohydrine, epoxybutane, styrene oxide and 1,2-epoxyhexane. 

MC Heller, JF Carpenter, TW Randolph. Effects of phase-separating systems on lyophilized hemoglobin. Journal of Pharmaceutical Sciences 85 1358-1362, 1996. 

Size - many bio-pharmaceutical research labs have severe constraints on the amount of bench space that can be made available for traditional analysis systems that typically required a bench-top PC to run them in addition to the similarly bulky detector itself, coupled in many cases to an even larger sample purification/separation system. In many cases, instrument size was also dictated by a desire on the part of manufacturers to add a premium market price. 

S. K. Poole, M. T. Belay and C. F. Poole, Effective systems for the separation of pharmaceutically important esti Ogens by tliin layer cltromatography , ]. Planar Chromatogr. 5 16-27 (1992). 

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