Phage libraries on purified immobilized

Selection of phage libraries on purified, immobilized antigen... 

The phage display library is now ready for use in an affinity selection protocol. Affinity selection has also been successfully utilized in vitro and ex vivo. The affinity selection principle is the same for all types of selection. First, allow the phage library to bind to the presented target. Wash away unbound phage, elute and amplify bound phage. Repeat the selection process three to four times. For example in vitro targets may be purified, biotinylated, and immobilized on a streptavidin-coated plate for affinity selection (9). 

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