Phage display material

To identify an individual enriched ligand from synthetic chemical libraries (see the chapter by Zhao and Lam), the number of variants used is limited by the amount of material which is needed for sequence analysis. The use of smaller beads has been made possible by inclusion of an encryption tag, e.g. an oligonucleotide, the sequence of which can be determined after PCR [44,45], The size of such banks is, all the same, similar to phage-display libraries with some 10s molecular species per ml, using 10 pm beads [46],... 

Ribosome Display. This cell-free display system uses the same principle as the phage-display systems in bacteria. The phenotype and genotype of a peptide are linked together and, therefore, can be simultaneously selected based on the function of the peptide. However, the linkage is a physical one in that the genetic material is covalently linked to its encoded product in the formation of an antibody-ribosome-mRNA (ARM) complex through in vitro transcription/translation [59]. 

The use of phage display was applied to the identification of a number of different peptides showing affinity for such materials as GaAs, ... 

We selected scFvs from a combinatorial phage display library using both phosphorylated and nonphosphorylated forms of a C-terminal y-H2AX peptide. Figure 2B shows die peptide sequence used for selection. Ten separate bacteriophage isolates were chosen for further characterization. Soluble scFvs were produced in the periplasm of an E. coli host. Periplasmic extracts were either used direcdy or as a source of material for further purification. 

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