Phage affinity

Rebar, E. J and Pabo, C O. (1994) Zinc finger phage affinity selection of fingers with new DNA-binding specificities. Science 263, 671-673... 

Phage affinity selection using cloned phage cDNA library I — I against biotinylated small... 

Cro, by contrast, acts purely as a repressor. When it is bound to its high-affinity site at OR3, it prevents repressor synthesis by obstructing the access of polymerase to the left-hand promoter. In the absence of repressor, RNA polymerase can bind to the Cro promoter, and Cro can be synthesized along with the early phage genes to its right. 

Affinity and specificity of proteinase inhibitors can be optimized by phage display... 

Katz, B. A. (1997). Structural and mechanistic determinants of affinity and specificity of ligands discovered or engineered by phage display. Armu. Rev. Biophys. Biomol. Struct. 26, 27-45. 

Vaughan, T. J., Williams, A. J., Pritchard, K., Osbourn, J. K., Pope, A. R., Eamshaw, J. C., McCafferty, J., Hodits, R. A., Wilton, J., and Johnson, K. S. (1996). Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nat. Biotechnol. 14, 309-314. 

Figure 16.4 Graph depicting the percentage of lysine residues among peptides that bind to the indicated monoclonal antibodies. The peptides were isolated after affinity selection (biopanning) from a phage-displayed combinatorial peptide library. The peptides are grouped as to whether they are susceptible to formalin fixation, resulting in a loss of immunoreactivity.

De Martino, T., Minenkova, O., Bombardi, V, Anastasi, A. M., Lindstedt, R., Felici, F., De Santis, R., Verdoliva, A. Identification and refinement of a peptide affinity ligand with unique specificity for a monoclonal anti-tenascin-C antibody by screening of a phage display library. / Chromatogr A 2006, 1107, 182-191. 

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