Pertussis Sources

Proteins of this class which have received the most attention were isolated from four bacterial species Pseudomonas aeruginosa, Ps. fluorescens, Ps. denitri-ficans, and Bordetella pertussis, although Sutherland (5) has isolated azurin from several other strains of Pseudomonas, Bordetella, and Alcaligenes. The near identity of azurins isolated from these different sources, has been generally assumed, and with one possible exception, that from Ps. denitrificans which does not bind to carboxy-methyl cellulose resin at the same pH as the other proteins (5), this seems to be the case. Ambler and Brown [6), who have elucidated the amino acid sequence of Ps. fluorescens azurin state that B. bronchiseptica, A. denitrificans and faecalis, Ps. denitrificans and fluorescens yield azurins having homologous amino acid sequences. 

One of the earliest effects found for PGEj was its ability to inhibit epinephrine-induced lipolysis in adipocytes by preventing the accumulation of cAMP ". The ability of PGEj to blunt isoproterenol-induced increases in cAMP in hamster adipocytes was subsequently shown to be reduced in hamsters that had been treated with a crude source of pertussis toxin. More recently, Murayama and Ui demonstrated that pertussis toxin treatment of adipocytes also diminishes the ability of PGEj to cause a GTP-dependent inhibition of adenylate cyclase activity in membranes prepared from these cells associated with this latter effect is a pertussis toxin-dependent, ADP-ribosylation of a protein subunit having the characteristic size (M = 41000) of the subunit of the inhibitory guanine nucleotide regulatory protein N. ... 

Source Reprinted with permission from Springer Science+Business Media, from Identification of respiratory pathogen Bordetella pertussis using integrated microfluidic chip technology, in Microfluid. Nanofluid. ... 

Table 5 [14] lists the acellular pertussis vaccine antibody response in mice for samples of Lot A of the DTaP Vaccine characterized by PT and FHA, sample control, and reference vaccine that had been irradiated (25kGy) using gamma, and X-ray sources. These data indieate that the irradiated vaccine gave equal or better antibody response eompared to the vaccine controls for the X-ray irradiated samples held at liquid nitrogen temperature during irradiation. 

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