Peroxylamine disulfonate

The intensity (or area) of a spectrum is calibrated with standards such as a, a -diphenyl-//-picrylhydrazyl (DPPH), potassium peroxylamine disulfonate [Fremy s salt K2NO(SO,)2], or carbon One gram of DPPH contains 1 5 X 1021 unpaired spins, and the strong pitch standard (0 1% powdered coal in KC1) has 3 x 1015 spins/cm of length A computer is useful for integrating the spectral area Alternatively, techniques have been developed m which the absorption line is cut out from the paper trace, weighed, and compared with that of a standard sample... 

The phenolic ring in estrone can be readily oxidized. Reaction of estrone with Fremy salts (peroxylamine disulfonate) affords a mixture of the two isomeric catechols. In a more controlled manner, treatment of estrone with 2-iodoxybenzoic acid (22-1) leads intially to a mixture of the 2,3-quinone (22-2) and its 3,4-isomer (22-3) (Scheme 3.22). These products are then reduced in situ with ascorbic acid to afford 2-hydroxyestrone (22-4) and 4-hydroxyestrone (22-5). 

Figure 17. Effect of modulation broadening on experimental ESR DISPA plots, (a) v-mode derivative ESR spectrum (mpO line) from 0.0005M peroxylamine disulfonate, using 13 mG modulation amplitude, 100 MHz modulation frequency, 5 mW power, swept at 1.0 G/min with a time constant of 0.125 sec. The "+" designates 3225 G. (b) DISPA plot from the data of (a), (c) DISPA plot as for (a), but with modulation amplitude = 0.2 G. (d) DISPA plot as for (a), but with modulation amplitude = 0.4 G. [Taken from ref. 3.]...

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