Peroxidase inhibitors

In order to preserve enzyme activity during the reaction process, special attention must be paid on the substrates that cause direct or indirect enzyme inactivation. Since peroxide is a strong peroxidase inhibitor, a low peroxide/enzyme ratio must be selected. When treating phenolic compounds, the polymeric products obtained from the action of peroxidases also cause enzyme inactivation [9]. If the enzyme is inactivated, not only is the reaction hindered but, sometimes, there is a direct oxidation of the substrate by the peroxide, which causes an enantioselective reduction in some synthetic reactions [10, 11]. In these cases, an appropriate enzyme concentration and usually an adequate enzyme addition strategy are considered [8],... 

An example of prediction results for sulfathiazole is shown in Figure 6.6. This substance was found in SAR Base and was excluded from the SAR Base on prediction of its activity spectrum. The known (contained in SAR Base of PASS version 2007) activity spectrum includes the following activities antibacterial, antibiotic, dihydropteroate synthase inhibitor, iodide peroxidase inhibitor. In Figure 6.6 the predicted activity spectrum includes 65 of 374 pharmacological effects, 176 of 2755 molecular mechanisms, 7 of 50 side effects and toxicity, 11 of 121 metabolism terms at default Pa> Pi cutting points. All activities included in the SAR Base are predicted with Pa> Pi- The activity of as... 

Dihydropteroate synthase inhibitor Iodide peroxidase inhibitor... 

Antioxidants, such as tea polyphenols, curcumin, and, camosol, may be expected to work by increasing intracellular GSH or total 011018, scavenging free radicals, or iron chelations. Many of the compounds classified as antioxidants that have been shown to inhibit NFkB activation are phytopolyphenols that are good peroxidase inhibitors or substrates. Many are effective at concentration that may be too low to be compatible with a radical-scavenging role, and their effects might be better explained if they were acting on a more specific enzymatic process. 

More than 100 NSAIDs are marketed or at an advanced stage of development worldwide. Their use is constantly expanding and the search for more efficacious and better-tolerated compounds is still being pursued, and has received renewed impulse with studies on selective inhibitors of cyclo-oxygenase type 2 (COX-2), nitric oxide-releasing NSAIDs, peroxidase inhibitors, enantiomers of already known NSAIDs, NSAIDs associated with zwitterionic phospholipids, and cytokine-modulating antirheumatic drugs. 

Straus W. Use of peroxidase inhibitors for immunoperoxidase procedures. In Feldman R, ed. Proceedings of the first international symposium on immunoenzymatic techniques. Amsterdam Elsevier 1976 117. 

Treat tissue sections with endogenous peroxidase inhibitor solution (4 min). 

Trostchansky, A., L. Bonilla, C. P Thomas et al. 2011. Nitroarachidonic acid, a novel peroxidase inhibitor of prostaglandin endoperoxide H synthases 1 and 2. 286... 

Isol. from the fungus F-124. Peroxidase inhibitor. Oil. Isol. as a mixture of cis- and trans-forms. 

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