Perkin-Elmer spectrophotometric detector

We studied conditions for the determination of tiametoxam (TM), the active component of the fungicide Actai a (Syngenta, Switzerland) by the method of thin layer chromatography with use of the Perkin-Elmer liquid chromatograph combined with spectrophotometric detector. The 250 mm-long and 4.6 mm in diameter steel column filled with Silasorb was used. 

Detection of A280nm Spectrophotometric Detector Model LC-75 (Perkin-Elmer, USA)... 

General. Commercially available polyimides were used (see Table I). Solvents were reagent grade or better and were used as received. 1-Methylpiperazine (Aldrich Chemical Co.) was > 99.9% pure. Lactic acid (Aldrich) was reduced to 50% by weight in water and refluxed for an hour to hydrolyze esters. IR spectra were recorded on a Perkin-Elmer Model 1430 spectrometer. Thermogravimetric analysis was done on a P-E System 4/TGS-2 instrument. Size exclusion chromatography was done on a Perkin-Elmer Series 3B equipped with the LC-75 spectrophotometric detector. The column set used consisted of P-E 0258-2134, 2133, and 2131 columns (pore sizes 103, 104, and 106 A, respectively). For electrophoretic deposition experiments, a TCR Power Supply (Electronic Measurement Systems Inc.) was used. Temperatures are reported in °C throughout. 

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