Perfused skin models

Further specimens in use are the perfused cow udder, the porcine forelimb, and the isolated perfused porcine skin flap (IPPSF) [55-57], While the first two require the sacrifice of the animal, the last one can be isolated surgically from the abdomen of the pigs, which afterwards can be returned to their prior disposition [49], 

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J. E. Riviere. Perfused skin models. In J. E. Riviere (ed.), Dermal Absorption Models in Toxicology and Pharmacology, Taylor Francis Group, Boca Raton, 2006. 

Isolated perfused skin models, such as the isolated perfused porcine skin flap (IPPSF) developed in our laboratory, may be the missing link in the hierarchy of classic in vitro and in vivo models. Primary advantages of isolated perfused systrans include the following ... 

The presentation here provides an overview of tlie uses of a perfused skin model such as the IPPSF in percutaneous absorption and dermatotoxieokinetie studies. One of its major advantages is that both absorption and toxicity may be assessed in the same preparation. The pharmaeokinetic models developed arc experimentally verifiable. The major limitations are centered on the cost of the preparation and the technical expertise required to successfully conduct the studies. The overall cost is significantly greater than in vitro diffusion cell studies or in vivo rodent experiments, comparable to human skin equivalent and larger mammal (dog, pig, primate) in vivo work and much less expensive than human trials. However, cost alone is not a sufficient criterion. These studies are humane more information may be gathered than is obtainable with either in vitro or in vivo work. Optimal benefit may be achieved if these studies serve as a bridge between in vitro human/animal and in... 

Monteiro-Riviere, N.A., 1992, Use of isolated perfused skin model in dermatotoxicology. In... 

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