Peptostreptococcus magnus protein

Graille M, Stura EA, Housden NG, Beckingham IA, Bottomley SP, Beale D, Taussig MI, Sutton BI, Gore MG, Charbonnier IB Complex between Peptostreptococcus magnus protein L and a human antibody reveals structural convergence in the interaction modes of Fab binding proteins. Structure 2001 9 679-687. 

SpA, Staphylococcal protein A PpL, protein L from Peptostreptococcus Magnus IgG, immunoglobulin G Gla, y-carboxyglutamic acid SPR, surface plasmon resonance ELISA, enzyme-linked immunosorbent assay FITC, fluorescein isothiocyanate NMR, nuclear magnetic resonance... 

Nilson BH, Solomon A, Bjorck L, Akerstrom B Protein L from Peptostreptococcus magnus binds to the kappa light chain variable domain. J Biol Chem 1992 267 2234-2239. 

Beckingham, J. A., Bottomley, S. P., Hinton, R., Sutton, B. J. and Gore, M. G, 1999, Interactions between a single immunoglobulin-binding domain of protein L from Peptostreptococcus magnus and a human k light chain. Biochemical Journal 340, 193-199. 

Figure 6 The binding cun/es obtained on mixing k chains with a mutated immunoglobulinbinding domain of protein L (SpL) from Peptostreptococcus magnus. The curves show the changes in protein fluorescence intensity from unique tryptophan residues (W39 or W64) as each domain binds to k chains at pH 8.0 in potassium phosphate buffer, 20X. The concentration of k chain was 3 pM and the concentration of each mutant of SpL was 30 pM. These data were kindly provided by Or J. Beckingham and N. Housden.

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