Peptidomics differential display

BioVisioN, Peptidomics, Differential Peptide Display and Spectromania are trademarks of BioVisioN AG, Hannover, Germany. [Pg.130]

Fig. (1). Peptidomics strategies used to study Drosophila immunity. (A) Using antimicrobial assays (antibacterial and antifungal), the bioactive peptides were isolated from the blood of bacteria-challenged Drosophila. MS was used for molecular mass assignment, to identify post-translational modifications, and for primary structure elucidation (B) Identification of peptidic immune effectors through differential display analysis (DD) by MALDI-MS and micro/nano RP-HPLC coupled (online) or not (off-line) to ESI-MS. When the HPLC was performed off-line to the mass spectrometer, fractions were individually analyzed by MALDI-MS. The identification and the structural characterization were performed either by molecular mass assignment and/or sequencing by ESI-MS/MS.

$Fig. (1). Peptidomics strategies used to study Drosophila immunity. (A) Using antimicrobial assays (antibacterial and antifungal), the bioactive peptides were isolated from the blood of bacteria-challenged Drosophila. MS was used for molecular mass assignment, to identify post-translational modifications, and for primary structure elucidation (B) Identification of peptidic immune effectors through differential display analysis (DD) by MALDI-MS and micro/nano RP-HPLC coupled (online) or not (off-line) to ESI-MS. When the HPLC was performed off-line to the mass spectrometer, fractions were individually analyzed by MALDI-MS. The identification and the structural characterization were performed either by molecular mass assignment and/or sequencing by ESI-MS/MS.$

Tammen, H., Schulte, I., Hess, R., Menzel, C., Kellmann, M., Mohring, T., Schulz-Knappe, P. (2005). Peptidomic analysis of human blood specimens comparison between plasma, specimens and serum by differential peptide display. Proteomics 5, 3414-3422. [Pg.219]

Schulz-Knappe P, Zucht HD, Schrader M. Differential peptide display. In Gaenschirt D, Harms F, Rohmann S, Schulz-Knappe P, editors. Peptidomics in Drug Development. Aulendorf, Germany Editio Cantor, 2005 33-35. [Pg.135]

Peptidomic analysis of human blood specimens comparison between plasma specimens and serum by differential peptide display. Proteomics 5, 414-422. [Pg.135]

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