Peptides recombinant human erythropoietin

Boss H J., Watson D.B., and Rush R.S. (1998), Peptide capillary zone electrophoresis mass spectrometry of recombinant human erythropoietin an evaluation of the analytical method, Electrophoresis 19(15), 2654—2664. [Pg.270]

Peptide mapping. (A) Peptide mapping of the international standard of rhEPO (recombinant human erythropoietin) (BRP - European Pharmacopea). (B) Peptide mapping of an rhEPO production lot. [Pg.564]

Darbepoetin alpha is a 165-amino acid protein that differs from recombinant human erythropoietin in containing five AFlinked oligosaccharide chains, whereas recombinant human erythropoietin contains three. The two additional AFglycosylation sites result from amino acid substitutions in the erythropoietin peptide backbone. The additional carbohydrate chains increase the approximate MW of the glycoprotein from 30,000 to 37,000 Da. [Pg.339]

Sasaki, H., Ochi, N., DeU, A., and Fukuda, M. (1988) Site-specific glycosyl-ation of human recombinant erythropoietin Analysis of glycopeptides or peptides at each glycosylation site by fast atom bombardment mass spectrometry. Biochemistry, 27 8618-8626. [Pg.345]