Peptide nucleic acid based biosensors

J. Wang, DNA biosensors based on peptide nucleic acid (PNA) recognition layers. A review. Biosens. Bioelectron. 13, 757-762 (1998). 

Endo, T., Kennan, K., Nagatani, N., Takamura, Y., Tamiya, E. (2005a). Label-free detection of peptide nucleic acid-DNA hybridization using localized surface plasmon resonance based optical biosensor. Anal Chem 77, 6976-6984. 

The ability to identify particular sequences of DNA, both quickly and precisely has become of increasing importance in recent years. Current DNA detection methods are limited by their need for the DNA in the sample to be chemically labelled before analysis. In this poster, the biosensor described does not. In fact, the detector is the DNA mimic, peptide nucleic acid (PNA) whilst the sensor is based on polydiacetylene (PDA) liposomes. It is envisaged that when the PNA detector binds to its target gene, the PDA sensor will be induced to change colour from blue to red. It is anticipated that this response will be detected visually and quantified through UVA/is spectroscopy. In order to improve the hydrophilic character of the liposomes, a hydrophilic spacer has been included. Here, we report our progress to date on the preparation and evaluation of PNA-functionalised PDA liposomes as potential, novel colorimetric nucleic acid biosensors. 

Chemosensors based on quinoline or fe(2-pyridyhnethyl)amine and biosensors based on peptides, proteins, and nucleic acids for fluorescent detection of zinc in biological systems 04CCR(248)205. 

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