Pentafluoropropionyl derivatives derivatizing

Heptafluorobutyrate derivatives are popular in isotope dilution reference methods for steroids such as testosterone [141], 17 -oestradiol [142-144] and progesterone [145-147]. Even difficult steroids such as aldosterone can be quantified in serum as the 3-enol-HFB ester by stable isotope dilution GC-MS [I4I] or after a new derivatization procedure with HFB anhydride [148). Pentafluoropropionyl derivatives have a)so found application to the quantification of oestriol [141] and urinary progesterone [149]. 

Amino acid enantiomers can be separated on a chiral stationary phase after derivatization with chloroformates (Abe et al., 1996). The derivatization procedure is quite simple and rapid, but the derivatizing reagent must be synthesized, which complicates the assay. Another method for the analysis of amino acid enantiomers uses N,0-pentafluoropropionyl isopropyl derivatives and a chiral column with NPD detection (Hashimoto et al., 1992). 

To be able to determine nicotine in sub-picomole quantities Neelakantan and Kostenbauer11 used an electron-capture detector in connection with derivatization of nicotine Nicotine was hydrogenated catalytically to yield N-methyl-4-(3 -piperidyl)-n-butylamine (= octahydro-nicotine), the two secondary amino-functions of which were treated with perfluoropropionic anhydride to give the di-pentafluoropropionyl octahydronicotine. The perfluoropropionyl derivative of octahydronicotine provides an electron capture derivative which represents a means of detecting nicotine in amounts of approximately l/100th of the minimum quantity of nicotine that is detectable by FID. 

---