Pentafluorophenyl bonded phase

S. L. Richheimer, M. C. Kent, and M. W. Bernart, Reversed-phase high-performance liquid chromatographic method using a pentafluorophenyl bonded phase for analysis of tocopherols,/. Chromatogr. A 677 (1994), 75-80. 

Another partial separation was achieved on an uncommon column material of Japanese origin (YMC-PACK A-302 S-5 ODS) eluted with propanol-water (35 65, v/v) (39). The most complete reversed-phase separation so far of p- and y-tocopherol is based on a pentafluorophenyl (PFPS) bonded phase and methanol-water as the eluent, as described by Richheimer (42) (Fig. 5). In their fundamental study on the separation of tocopherols on reversed phases, Abidi and Mounts corroborated the results of Richheimer with respect to the superiority of the PFPS phase for the separation of positional isomers, including 5,7-dimethyl-... 

Solid-phase techniques can be applied to the N-activated esters.16 In the activated ester method, 4-nitrophenyl, 12 21-27 2,4-dinitrophenyl, 614 28 2,4,5-trichlorophenyl, 29 and penta-fluorophenyl 8 esters can be used, the amide bonds being formed with aminolytic release of the corresponding phenol (Scheme 4). For example, the use of pentafluorophenyl esters in the synthesis of pure azapeptides (named azatides for the first time) proved to be advantageous. 8 ... 

In this case study, two different Cl 8 columns from different manufacturers were used. Alternatively, other stationary phase types could also be used such as a polar embedded phase and a Cl 8 phase. Some systems come also equipped with a six-column switcher and in that case, two different types of polar embedded phases, phenyl phase, pentafluorophenyl phases, two different Cl 8 phases (of different bonding density) and an alternate C8 phase could be used. 

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