Peanut lectin isolation

Fischer, J., Klein P.-J., Farrar, G. H., Hanisch, F.-G. and Uhlenbruck, G. 1984. Isolation and chemical and immunological characterization of the peanut-lectin-binding glycoprotein from human milk-fat-globule membranes. Biochem. J. 224, 581-589. 

One approach to this problem would be to isolate the intact lectin-receptor molecules from the cell membranes and characterize them. Work in this direction has been initiated in our laboratory, and a method for the isolation of the peanut agglutinin receptor from membranes of neuraminidase-treated human erythrocytes on a column of peanut agglutinin-polyacryl-hydrazido-Sepharose has been developed (21). The amino acid composition, D-glucosamine ancHg-galac-tosamine content, and the electrophoretic mobility on polyacrylamide gel electrophoresis in sodium... 

Another important assumption from the lectin binding studies was that hemopoietic stem cells in the mouse may carry receptors for both peanut agglutinin and soybean agglutinin. Evidence in support of this assumption was obtained when, with the aid of peanut agglutinin and soybean agglutinin a cell fraction was isolated from mouse bone marrow and spleen, which was enriched with hemopoietic stem cells and depleted of "graft-versus-host" activity. Furthermore, such a cell fraction could be used for successful reconstruction of irradiated allogeneic mice (3SH. ... 

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