Peak purity assessment

In some cases a principal components analysis of a spectroscopic- chromatographic data-set detects only one significant PC. This indicates that only one chemical species is present and that the chromatographic peak is pure. However, by the presence of noise and artifacts, such as a drifting baseline or a nonlinear response, conclusions on peak purity may be wrong. Because the peak purity assessment is the first step in the detection and identification of an impurity by factor analysis, we give some attention to this subject in this chapter. 

F.C. Sanchez, J. Toft, B. van den Bogaert and D.L. Massart, Orthogonal projection approach applied to peak purity assessment. Anal. Chem., 68 (1996) 79-85. 

P. Zhang and D. Littlejohn, Peak purity assessment by matrix projection for spectral line selection and background correction in inductively coupled plasma optical emission spectrometry, Spectrochim. Acta, Part B, 50(10), 1995, 1263-1279. 

Castledine, J.B. Fell, A.F. Stategies for peak-purity assess- 39. ment in liquid chromatography. J. Pharm. Biomed. Anal. 

Castledine, J. B. and Fell, A. F. Strategies for peak purity assessment in liquid chromatography. /. Pharm. Biomed. Anal., 11 1—13, 1993. 

The use of diode-array detection has greatly improved peak identification, peak purity assessment and quantification. However, there are still some problems to be solved relating to peak purity assessment (compounds possessing identical spectral characteristics may invalidate peak purity criteria) and peak quantification (poor resolved peaks cannot be quantified by the... 

Figure 9.5. Diagram illustrating peak purity assessment using UV and MS. Panel (a) shows that since the UV spectra of the leading, apex, and trailing parts of the first peak look fairly similar visually, one might conclude erroneously that the first peak contains only one single component. Panel (b) shows that the first peak actually contains two components with masses at 311 and 287, respectively. These two components might have similar UV spectra, making UV spectroscopy an insensitive tool for peak purity evaluation. Diagram courtesy of Waters Corporation.

Cameron G, Jackson PE, Gorenstein MV (1993) A new approach to peak purity assessment using photodiode array detection. Chem Aus, 288-289. 

Review excipient compatibility Peak purity assessment as... 

Mass balance and peak purity assessment completed Isolation, mechanistic... 

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