Pathogen Sensing Using Immunoassays

Immunoassay platforms, where metallic NPs are functionalized with antibodies or aptamers that recognize target biomolecules on the surface of the pathogen, represent another approach to detect pathogens. The main principles of immunoassays have been explained in Sect. 5.3.3. 

Knauer and co-workers reported the label-free SERS detection of Legionella pneumophila and Salmonella typhimurium cells in water (Knauer et al. 2010). The total assay time was only 65 min. The bacterial suspension was incubated on a glass chip containing the respective antibodies. After the cells were captured and 

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