Particulate-based proliposomes

Large-Scale Manufacture of Particulate-Based Proliposomes.411... 

ProUposome technologies have been introduced as a potentially applicable resolution to the stability problems of liposomes. Proliposomes are phospholipid preparations that generate liposomes by addition of aqueous phase and shaking. Proliposomes can be classified into two types particulate-based proliposomes [48] and solvent-based proliposomes [49],... 

Particulate-based proliposomes are soluble carrier (e.g., carbohydrate) particles coated with phospholipids. The addition of aqueous phase results in the formation of MLVs [48,50], On a small scale, particulate-based proliposomes are made by placing the carrier particles in a round-bottomed flask. The flask is attached to a modified rotary evaporator with feed line. A chlorofonnic solution of lipid is coated dropwise onto the carrier particles via the feed line under reduced pressure. Solvent evaporation produces proliposomes which can be either hydrated innnediately by addition of aqueous phase, or stored in the freezer for future use. 

Zhang and Zhu [60] have combined two methods in preparing liposomes, by hydrating particulate-based proliposomes followed by freeze-drying. This approach has been reported to enhance the entrapment of amikacin compared to the same vesicles before freeze-drying or vesicles prepared by the thin film method. 

Large-scale manufacture of particulate-based proliposomes 411... 

LARGE-SCALE MANUFACTURE OF PARTICULATE-BASED PROLIPOSOMES... 

Table 15.1 Entrapment Efficiency of Drugs in Liposomes Generated from Particulate-Based Proliposomes...

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