Parathion hydrolase, gene

Mulbry WW, JS Karns, PC Kearney, JO Nelson, CS McDaniel, JR Wild (1986) Identification of a plasmid-borne Parathion hydrolase gene from Flavobacterium sp. by Southern hybridization with opd from Pseudomonas diminuta. Appl Environ Microbiol 51 926-930. 

Sedar, C. M., Gibson, D. T. (1985). Enzymatic hydrolysis of organophosphates Cloning and expression of a parathion hydrolase gene from Pseudomonas diminuta. Bio/Technology, 3, 567-571. 

A parathion hydrolase has been described to hydrolyze both phosphorothioates and phosphates and its gene, opd, cloned from a plasmid carried by Psudomonas diminuta... 

In addition, a leader peptide encoded by the opd gene functions to insert the processed parathion hydrolase protein in the membrane of the native Flavobacterium and Pseudomonas diminuta strains but causes the protein to be excreted by Streptomyces lividans (26,28). The ability of these regulatory elements to function across such a wide range of microorganisms is strongly suggestive of the important role that plasmid mobility and recombination have played in the dissemination of the opd gene. 

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