Paramagnetic microbeads

Streptavidin-coated super-paramagnetic microbeads (Miltenyi Biotech). 

Resuspend the cell pellet in 2.5 mL PBS and add 40 pL streptavidin-coated super-paramagnetic microbeads. Incubate for 15 min on ice. Mix gently every... 

Our first successful effort at miniaturizing assays came with capillary-based immunoassays. More recently, we have developed several miniaturized immunoassays using paramagnetic microbeads to serve as the heterogeneous surface. Currently we are investigating different automated electrochemical im-munosensor systems with the aim of extending ECIA to lab-on-a-chip systems. In the remainder of this chapter, we wiU present the accomplishments in each of these areas. 

Vidal, J.C., Bond, L., Ezquerra, A. et al. (2012) An electrochemical immunosensor for ochratoxin A determination in wines based on a monoclonal antibody and paramagnetic microbeads. Ana/. Bioanal. Chem., 403, 1585-1593. Fernandez-Baldo, M.A., Bertolino, F.A., Fernandez, G. et al. (2011) Determination of ochratoxin A in apples contaminated with Aspergillus ochraceus by using a microfluidic competitive immunosensor with magnetic nanoparticles Analyst, 136, 2756-2762. 

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