Parallel preload

In parallel, preload the wells of two 96-well U-bottom plates with chemokines at various concentrations in a vol of 100 pL. Chemokines are diluted in the same... 

Diversity Sciences developed a library synthesis strategy that combines the simplicity of parallel synthesis and the power of resin-mixing techniques. The general format is four 96-well plates that give rise to 384 synthetic wells, as shown in Figure 8.9. The layout of the synthesis blocks enables 16 unique monomers in monomer position A (across rows) and 24 unique monomers in monomer position B (down the columns). All of the 384 wells are preloaded with off-the-shelf resin where each well has a unique binary code embedded in the analytical construct. The first two points of diversity (monomer A and monomer B) is added in all possible combinations by parallel synthesis. Each spatial location has a unique binary-mass code that encodes for a particular combination of monomer A and monomer B. For example, binary code number 8 represents monomer Al and monomer B8. After the addition of monomer B, the resin from all 384 wells is mixed together and split into 96 identical pools, to which monomer C is added. The third monomer, monomer C, is spatially encoded, since the 96 pools are not mixed after the last step and screened as pools. Upon decoding, the identification of the binary code reveals the combination of monomer A and monomer B on each bead. 

The fact that Grlgnard compounds are used for measurement of hydroxyl content of silica can be recalled to confirm this appreciation (23). Nevertheless, it can not be totally ruled out that some hydroxyl groups remain still unreactlve after BxjMgCl treatment. In the second step, the Mg modified silicas were reacted with an excess of TiCl in n-heptane. Different temperatures and reaction times were also employed to carry out this reaction. The amount of supported TiCl tends to be parallel to the amount of preloaded Mg. The Ti/Mg ratios obtained with the four silicas are given in Table II. These results can be explained by admitting that clusters of TiCl were formed surrounding the Mg atoms, as formulated in scheme shown in Fig. 2. 

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