Pair reversals

Amin, M., Simultaneous determination of prostaglandins (PG) E2, A2 and B2 and stability studies of PGE2 in pharmaceutical preparations by ion-pair reversed phase HPLC, Pharm. Acta. Helv., 64, 45, 1989. 

Toll, H., Oberacher, H., Swart, R., Huber, C.G. (2005). Separation, detection, and identification of peptides by ion-pair reversed-phase high-performance hquid... 

Kamei et al. [45] separated spermine, spermidine, putrescine, and cadav-erine in an ion-pair reversed-phase LC system and detected the hydrogen peroxide formed in the reaction catalyzed by the enzymes putrescine oxidase and polyamine oxidase with POCL. The same analytes were determined in a later study [46], together with the acetyl derivatives. The sensitive determination of uric acid, selectively converted to hydrogen peroxide by uricase, has been investigated by several authors [37, 47],... 

A combination of different modes of chromatography can provide excellent resolution for those components that chromatograph poorly with a single mode [108,109]. The chromatographic separation of baclofen (I) from its potential transformation product (II) with dual mode chromatography entailing ion-pair reversed-phase chromatography and... 

S. Ahuja, Retention Mechanism Investigations on Ion-pair Reversed Phase Chromatography, American Chemical Society meeting, Miami, April 28, 1985. 

A.R. Bahrami, M.J. Dickman, M.M. Matin, J.R. Ashby, P.E. Brown, M.J. Conroy, G.J.S. Fowler, J.P. Rose, Q.I. Sheikh, A.T. Yeung and D.P. Hornby, Use of fluorescent DNA-intercalating dyes in the analysis of DNA via ion-pair reversed-phase denaturing high-performance liquid chromatography. Anal. Biochem., 309 (2002) 248-252. 

K.J. Fountain, M. Gilar, Y. Budman and J.C. Gebler, Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography. J. Chromatogr.B, 783 (2003) 61-72. 

W.R. Melander and Cs. Horvath, Mechanistic study of ion-pair reversed-phase chromatography , J. Chromatogr., 1980, 201, 211. 

Aboul-Enein and Al-Duraibi (1998) employed dansyl chloride in a fluorescence assay for PUT, SP, SPD, and their acetylated derivatives by ion-pair reverse-phase chromatography. This assay could be applied to the separation of free and acetylated polyamines in biological samples. Dansyl chloride has also been used as the fluorescence reagent in the determination of polyamines in urine by Molins-Legua and colleagues (1999). Derivatization was carried out within the C18 cartridges that were used during the SPE extraction procedure. Recoveries were 80-95% for all four polyamines analyzed and the hmit of detection was 10 ng/ml. 

Ion-pair reverse phase HPLC. Aqueous samples confaining pyridoxine as infernal standard and 0.5% heptafluorobutyric acid as ion-pairing agent (IP) were injected in an RP-HPLC system with a Varian ODS-80TM column and eluted with 0.15% heptafluorobutyric acid in 24% methanol. Pyridinoline cross-link fluorescence was defecfed af Xgx 295 nm and Xem 400 nm (Bank ef al., 1997). 

The elucidation of the retention mechanism in ion-pair reversed-phase chromatography using alkyl amines or alkyl sulfonates as hetaerons has evoked significant interest not only for the great potential of the method in the separation of ionic compounds but also for theoretical reasons. 

Dai, G., Wei, X., Liu, Z., Liu, S., Marcucci, G., and Chan, K. K., Characterization and quantification of Bcl-2 antisense G3139 and metabolites in plasma and urine by ion-pair reversed phase HPLC coupled with electrospray ion-trap mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 825(2), 201-213, 2005. 

B. Do, S. Robinet, D. Pradeau and F. Guyon, Speciation of arsenic and selenium compounds by ion-pair reversed-phase chromatography with electrothermal atomic absorption spectrometry. Application of experimental design for chromatographic optimisation, J. Chromatogr. A, 918(1), 2001, 87-98. 

E Verdon, P Couedor. Determination of ampicillin residues in milk by ion-pair reversed-phase high-performance liquid chromatography after precolumn derivatization. J Pharmaceut Bionied Anal 14 1201-1207, 1996. 

W Luo, EB Hansen Jr, CYW Ang, HC Thompson Jr. Determination of lincomycin residue in salmon tissues by ion-pair reversed-phase liquid chromatography with electrochemical detection. J AOAC Int 79 839-843, 1996. 

CEC was used to separate double-stranded DNA (dsDNA) on microchips [58,152]. In these cases, Ci8-modified PMMA microchips were employed with ion-pair reverse phase chromatography and DNA fragments with different sizes were analysed by contact conductivity detection. 

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