P-hydroxydecanoyl-ACP dehydrase

Isomerization of monoenes occurs in microorganisms for a number of reasons. One of these is because isomerization is an essential step in the synthesis of unsaturated fatty acids by anaerobes. A specific dehydrase (P-hydroxydecanoyl-ACP dehydrase) is capable of both dehydration of a 10-carbon intermediate in a growing fatty acyl chain as well as its isomerization from a trans-2 decanoyl-ACP to a af-d-decenoyl-ACP (32). The anaerobic pathway of unsaturated fatty acid synthesis accounts for 1-6% oleic acid in ruminal microorganisms (33). Other anaerobes, such as Streptococcus pneumoniae, isomerize a trans-2 decanoyl-ACP to a r-3-decenoyl-ACP during fatty acid synthesis without catalyzing the dehydration of P-hydroxy intermediates (34). 

CAN E. COLI p-HYDROXYDECANOYL-ACP DEHYDRASE AND P-KETO-ACYL-ACP SYNTHASE I INTERACT WITH BRASSICA NAPUS FATTY ACID SYNTHASE TO ALTER OIL SEED COMPOSITION ... 

Birge, C.H., Silbert, D.F. and Vagelos, P.R. (1967) A p-hydroxydecanoyl-ACP dehydrase speeifie for saturated fatty acid biosynthesis in. coli. Biochem. Biophys. Res. Commun. 29 808-814. 

Can E.coli p-Hydroxydecanoyl-ACP Dehydrase and P-Keto-Acyl-ACP Synthase I Interact with Brassica napus Fatty Acid Synthase to Alter Oil Seed Composition ... 

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